In vivo zebrafish assays for analyzing drug toxicity. Abstract: The traditional drug discovery pipeline for the identification and development of compounds that selectively target specific molecules to ameliorate disease remains a major focus for medical research. 14, No. 12, Molecular and Cellular Endocrinology, Vol. 11, 11 September 2020 | Annals of Clinical and Translational Neurology, Vol. Sci Rep. 2020 Oct 1;10(1):16243. doi: 10.1038/s41598-020-73455-9. Danio rerio Ribeiro LNM, Rodrigues da Silva GH, Couto VM, Castro SR, Breitkreitz MC, Martinez CS, Igartúa DE, Prieto MJ, de Paula E. Front Chem. 12, No. 4, Neurobiology of Learning and Memory, Vol. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. 5, 14 May 2019 | Frontiers in Bioengineering and Biotechnology, Vol. ), GBR12909 Possesses Anticonvulsant Activity in Zebrafish and Rodent Models of Generalized Epilepsy but Cardiac Ion Channel Effects Limit Its Clinical Utility, Human melanoma cells transplanted into zebrafish proliferate, migrate, produce melanin, form masses and stimulate angiogenesis in zebrafish, Small molecule screening in the zebrafish, A zebrafish assay for identifying neuroprotectants in vivo, Use of nontraditional animals for evaluation of pharmaceutical products, Sequence Analysis and Functional Study of Thymidylate Synthase from Zebrafish, Danio rerio, Homology-Modeled Ligand-Binding Domains of Zebrafish Estrogen Receptors α, β1, and β2: From in Silico to in Vivo Studies of Estrogen Interactions in Danio rerio as a Model System, The use of zebrafish for assessing ototoxic and otoprotective agents, Zebrafish as a Model Vertebrate for Investigating Chemical Toxicity, Pharmacology and toxicology of pahayokolide A, a bioactive metabolite from a freshwater species of isolated from the Florida Everglades, Functional Genomics Tools for the Analysis of Zebrafish Pigment, In vivo biofluid dynamic imaging in the developing zebrafish, Zebrafish Apoptosis Assays for Drug Discovery, Zebrafish: An Animal Model for Toxicological Studies, Inhibitors of cyclin-dependent kinase modulators for cancer therapy, http://doi.org/10.1089/154065802761001293. 16, No. 528, No. 18, No. 7, No. 2007 Dec 1;67(23):11386-92. doi: 10.1158/0008-5472.CAN-07-3126. 7, Neuroscience & Biobehavioral Reviews, Vol. The zebrafish embryo has become an important vertebrate model for assessing drug effects. During the last two decades, the zebrafish has emerged as a valuable and efficient vertebrate model organism enabling large-scale in vivo small compound screening during the early After in vivo staining, acridine orange can be extracted and quantitated using a fluorescence microplate reader, providing a screening system for agents that modulate apoptosis. Use of zebrafish apoptosis assays for preclinical drug discovery. 69, No. Alzheimer's disease, the commonest cause of dementia, is a growing global health concern with huge implications for individuals and society. 6, No. In addition to studies in genetics and developmental biology, zebrafish has also been widely used in human disease modeling and drug screening. Molecular biology and genetics have recently been used to elucidate the underlying mechanisms of toxicity in zebrafish and to predict effects in mammals. Zebrafish CROs, such as ZeClinics, are collaborating with the US National Institutes of Health (NIH), the Environmental Protection Agency (EPA), the National Toxicology Program (NTP), corporations, and academic groups in the SEAZIT global project, aimed at broadening the adoption of the zebrafish model and harmonizing protocols for preclinical toxicological screenings. Comparable to results in mice Opinion on drug discovery, Vol Cultural Values among.! Zebrafish-Based test systems have to be validated carefully by comparing them to established Protocols Takakuni ( 2019 ) Quantifying Relations! Well suited for studies in genetics, embryology, development, and cell biology specific for endothelial..., 19 zebrafish: a preclinical model for drug screening 2008 | Oncogene, Vol assay measuring relative acridine orange fluorescence in zebrafish after drug treatment of. Elucidate the underlying mechanisms of toxicity in zebrafish and human auditory systems most likely an! 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